Jane Tregunno got her start with the Canadian national rowing team in 1978, when she won a bronze medal in the coxed eights at the 1978 Junior World Championships. She upgraded to gold the following year and was selected to compete at the 1980 Summer Olympics, but stayed home after Canada joined the boycott of those Games. In 1981 she placed fourth in the coxed eights event at the Senior World Championships, alongside Heather Clarke, Vicki Harber, Cathy Lund, Andrea Schreiner, Lesley Thompson-Willie, Kay Worthington, and the non-Olympians Jane Pringle and Sylvia Wetzl. She switched to the coxed fours for the 1983 edition and finished fourth with Thompson-Willie, Barbara Armbrust, Gail Cort, and Kathey Lichty before attending the 1984 Summer Olympics, where she won a silver medal alongside Armbrust, Thompson-Willie, Marilyn Brain, and Angela Schneider. She then captured gold and bronze medals in the event at the 1986 Commonwealth Games and World Championships respectively, with Thompson-Willie, Christine Clarke, Tricia Smith, and Jennifer Walinga. This quintet, with the non-Olympian Kathryn Barr in place of Smith, came in sixth at the 1987 World Championships, and then joined Heather Clarke, Schreiner, Lisa Robertson , and the non-Olympian Maureen Cureton to finish seventh in the coxed eights at the same tournament. Tregunno’s final major international tournament was the 1988 Summer Olympics, where her 1987 crew placed seventh in the coxed fours. She retired from active competition after the Games and, as of 2013, works as an accountant.
Canada