| Date | 4 – 5 August 1928 |
|---|---|
| Status | Olympic |
| Location | Olympisch Stadion, Amsterdam |
| Participants | 32 from 8 countries |
The Canadian team ran an unratified world record of 49.3 in the heats. In the final, the US team took the early lead, but Canada pulled even at the first exchange. With the US having Betty Robinson, the 100 metre gold medalist, on the anchor leg, the Americans had some hope, but Myrtle Cook took the baton with a three-metre lead and she held on comfortably to give Canada a gold medal in world record time.
| Date | 4 August 1928 — 14:45 |
|---|---|
| Format | Top 3 in each heat advanced to the final round. |
| Pos | Lane | Competitors | NOC | Time (Hand) | ||
|---|---|---|---|---|---|---|
| 1 | 4 | Canada |  CAN | 49.3 | ||
| Lead-Off | 677 | Bobby Rosenfeld | – | |||
| 2nd Leg | 678 | Ethel Smith | – | |||
| 3rd Leg | 674 | Jane Bell | – | |||
| Anchor | 675 | Myrtle Cook | – | |||
| 2 | 1 | Netherlands |  NED | 50.4 | ||
| Lead-Off | 339 | Lies Aengenendt | – | |||
| 2nd Leg | 340 | Rie Briejèr | – | |||
| 3rd Leg | 346 | Nettie Grooss | – | |||
| Anchor | 348 | Bets ter Horst | – | |||
| 3 | 2 | France |  FRA | 51.0 | ||
| Lead-Off | 118 | Georgette Gagneux | – | |||
| 2nd Leg | 124 | Yolande Plancke | – | |||
| 3rd Leg | 125 | Marguerite Radideau | – | |||
| Anchor | 126 | Lucienne Velu | – | |||
| 4 | 3 | Sweden |  SWE | 53.2 | ||
| Lead-Off | 872 | Maud Sundberg | – | |||
| 2nd Leg | 870 | Inga Gentzel | – | |||
| 3rd Leg | 871 | Emy Pettersson | – | |||
| Anchor | 873 | Ruth Svedberg | – |
| Pos | Lane | Competitors | NOC | Time (Hand) | ||
|---|---|---|---|---|---|---|
| 1 | 4 | United States |  USA | 49.8 | ||
| Lead-Off | 588 | Mary Washburn | – | |||
| 2nd Leg | 573 | Jessie Cross | – | |||
| 3rd Leg | 578 | Loretta McNeil | – | |||
| Anchor | 879 | Betty Robinson | – | |||
| 2 | 2 | Germany |  GER | 49.8 | ||
| Lead-Off | 757 | Rosa Kellner | – | |||
| 2nd Leg | 764 | Leni Schmidt | – | |||
| 3rd Leg | 755 | Anni Holdmann | – | |||
| Anchor | 756 | Leni Junker | – | |||
| 3 | 3 | Italy |  ITA | – | ||
| Lead-Off | 243 | Luigia Bonfanti | – | |||
| 2nd Leg | 246 | Giannina Marchini | – | |||
| 3rd Leg | 247 | Derna Polazzo | – | |||
| Anchor | 248 | Vittorina Vivenza | – | |||
| 4 | 1 | Belgium |  BEL | – | ||
| Lead-Off | 640 | Elise Van Truÿen | – | |||
| 2nd Leg | 632 | Rose Van Crombrugge | – | |||
| 3rd Leg | 637 | Juliette Segers | – | |||
| Anchor | 638 | Léontine Stevens | – |
| Date | 5 August 1928 — 14:00 |
|---|
| Pos | Lane | Competitors | NOC | Time (Hand) | ||
|---|---|---|---|---|---|---|
| 1 | 1 | Canada | CAN | 48.4 | ||
| Lead-Off | 677 | Bobby Rosenfeld | – | |||
| 2nd Leg | 678 | Ethel Smith | – | |||
| 3rd Leg | 674 | Jane Bell | – | |||
| Anchor | 675 | Myrtle Cook | – | |||
| 2 | 3 | United States | USA | 48.8 | ||
| Lead-Off | 588 | Mary Washburn | – | |||
| 2nd Leg | 573 | Jessie Cross | – | |||
| 3rd Leg | 578 | Loretta McNeil | – | |||
| Anchor | 879 | Betty Robinson | – | |||
| 3 | 4 | Germany | GER | 49.0 | ||
| Lead-Off | 757 | Rosa Kellner | – | |||
| 2nd Leg | 764 | Leni Schmidt | – | |||
| 3rd Leg | 755 | Anni Holdmann | – | |||
| Anchor | 756 | Leni Junker | – | |||
| 4 | 6 | France | FRA | 49.6 | ||
| Lead-Off | 118 | Georgette Gagneux | – | |||
| 2nd Leg | 124 | Yolande Plancke | – | |||
| 3rd Leg | 125 | Marguerite Radideau | – | |||
| Anchor | 126 | Lucienne Velu | – | |||
| 5 | 5 | Netherlands | NED | 49.8 | ||
| Lead-Off | 339 | Lies Aengenendt | – | |||
| 2nd Leg | 340 | Rie Briejèr | – | |||
| 3rd Leg | 346 | Nettie Grooss | – | |||
| Anchor | 348 | Bets ter Horst | – | |||
| 6 | 2 | Italy | ITA | 53.6 | ||
| Lead-Off | 243 | Luigia Bonfanti | – | |||
| 2nd Leg | 246 | Giannina Marchini | – | |||
| 3rd Leg | 247 | Derna Polazzo | – | |||
| Anchor | 248 | Vittorina Vivenza | – |
